Life Sciences Division
1997-98
Progress Report
- Overview
- Mission
- Structure
- Lab Animal Resources
- Operations & Support
- Mouse Genetics Research
- DNA Sequencing Lab
- Gene Expression Chip Lab
- Dosimetry & Biokinetic
- Life Cycle Analysis
- Environmental Assessment
- Biological Sciences
- Functional Genomics & Proteomics
- Structural Biology
- Biomedical Engineering
- Technology Applications
DNA Sequencing Lab
A DNA sequencing and automated genotyping laboratory has been established at ORNL. This laboratory can provide researchers in Life Sciences and other Laboratory divisions with state-of-the-art DNA sequencing and automated fluorescence genotyping. The current facility is housed in approximately 1395 sq. ft. of lab and office space. Sequencing equipment currently consists of 1 ABI 377 sequencing machine (modified for 96 lanes) and 1 ABI 373 sequencing machine (modified for 64 lanes). For template preparation the lab has a Qiagen template robot capable of producing 96 sequence-ready DNA templates in less than 2 hours. Recently, in collaboration with the Robotics and Process Systems Division, we have constructed a robotic line for carrying out DNA sequencing reactions. This line integrates a CRS A465 robotic arm with a Robbins Hydra pipettor (96 well), MJR quad PCR machine, and a refrigerator (with actuator door). Associated with the DNA sequencing facility is a standard molecular biology lab which includes a Beckman Ultra centrifuge L8-80M, BioRad electroporater, 2 New Brunswick air shakers, microfuges, various gel electrophoresis equipment, etc. Computer needs of the facility are taken care of by a group of networked computers including 3 PowerPCs, 7 PCs, and 1 SGI O2 machine.
Contact: Richard J. Mural, 865-576-2938, e-mail: m9l@ornl.gov